Prophase in meiosis series#

The reorganization of the nuclear contents, and their situation with respect to the nuclear envelope, are, quite understandably, critical to the success of homolog alignment and, as a consequence, are essential for prophase I progression. One of the fascinating aspects of meiotic prophase I is the delicate, yet dramatic, orchestrated вЂњdanceвЂќ that occurs between homologous chromosomes. Kim Holloway, in Knobil and Neill's Physiology of Reproduction (Fourth Edition), 2015 Chromosome Movements, Telomere Clustering, and Telomere Bouquet Formation Second, injection of recombinant MSP can restore meiotic maturation in females. Two lines of evidences suggest that MSP is indeed the sperm-derived factor that triggers oocyte maturation ( Miller et al., 2001): First, injection of anti-MSP antibody reduced ovulation rate in wild-type worms. They then fractionated sperm-conditioned medium by HPLC and analyzed a fraction that retained its activity by MALDI-TOF mass spectrometry, and identity of the active component was revealed as MSP.

Prior incubation of the sperm-conditioned medium with protease abolished its ability to trigger meiotic maturation and gave them the clue that the active component is protein. The female worms supplemented with sperm-conditioned medium underwent normal meiotic maturation. let the isolated sperm stay in a buffer for few hours and injected the cell-free supernatant (sperm-conditioned medium) into the uterus of female worms.

Prophase in meiosis series#

performed a series of experiments and found that MSP is the sperm-derived factor that triggers meiotic maturation in oocytes ( Miller et al., 2001). What component in the sperm triggers meiotic maturation in oocytes? Toward answering this question, Miller et al. When the sperm are supplied to these spermless worms through males, the oocytes resume meiosis and undergo normal meiotic maturation.

In mutant worms that do not produce any sperm, the oocytes are arrested for prolonged period in meiotic prophase I. The resumption of meiosis depends on the cues from sperm. Oocytes are arrested in meiotic prophase I in many animals, including C. Gunasekaran Singaravelu, Andrew Singson, in International Review of Cell and Molecular Biology, 2011 5.1 MSP triggers oocyte maturation